Monoclonal Anti-NPPB antibody produced in mouse
SIGMA/WH0004879M1 - clone 2D11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BNP; Anti-natriuretic peptide precursor B
MDL Number: MFCD01321777
Product Type: Chemical
Western Blotting Western Blot analysis of NPPB expression in transfected 293T cell line by NPPB monoclonal antibody, clone 2D11. Lanes Lane 1: NPPB transfected lysate (14.726 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (40.48 kDa).
Immunoprecipitation Immunoprecipitation of NPPB transfected lysate using anti-NPPB monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with NPPB rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC025785   |
| isotype | IgG3κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P16860 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein′s biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. (provided by RefSeq) |
| Immunogen: | NPPB (AAH25785, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDPQTAPSRALLLLLFLHLAFLGGR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |