Monoclonal Anti-OCRL antibody produced in mouse
SIGMA/WH0004952M2 - clone 4A6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-LOCR; Anti-NPHL2; Anti-OCRL1; Anti-
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to OCRL on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of OCRL expression in transfected 293T cell line by OCRL monoclonal antibody, clone 4A6. Lanes Lane 1: OCRL transfected lysate (103.2 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of OCRL over-expressed 293 cell line, cotransfected with OCRL Validated Chimera RNAi. Blot probed with OCRL monoclonal antibody, clone 4A6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting OCRL monoclonal antibody, clone 4A6 Western Blot analysis of OCRL expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (36.08 kDa).
ELISA Detection limit for recombinant GST tagged OCRL is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_000276   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q01968 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. (provided by RefSeq) |
| Immunogen: | OCRL (NP_000267, 146 a.a. ~ 239 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SGLLGFEDNFSSMNLDKKINSQNQP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |