Monoclonal Anti-SERPINE1 antibody produced in mouse
SIGMA/WH0005054M1 - clone 3F2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PAI; Anti-PAI1; Anti-PLANH1; Anti-serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
MDL Number: MFCD00164749
Product Type: Chemical
Western Blotting Western Blot analysis of SERPINE1 expression in transfected 293T cell line by SERPINE1 monoclonal antibody, clone 3F2. Lanes Lane 1: SERPINE1 transfected lysate (45.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
Immunoprecipitation Immunoprecipitation of SERPINE1 transfected lysate using anti-SERPINE1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with SERPINE1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged SERPINE1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_000602   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P05121 |
| Application: | Monoclonal Anti-SERPINE1 antibody has been used in immunoblotting. |
| Biochem/physiol Actions: | PAI-1(plasminogen activator inhibitor) blocks tissue-type (tPA) and urokinase (uPA) plasminogen activators (PAs). It controls the flow of blood, hence PAI-1 is exposed to strict modulation at the genetic and protein level. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SERPINE1 (serpin family E member 1) is coded by PAI-1(plasminogen activator inhibitor) gene. It is a serine protease inhibitor. It is predominantly expressed in the vascular endothelial cells. This gene is mapped to human chromosome 7q22. |
| Immunogen: | SERPINE1 (NP_000593, 86 a.a. ~ 185 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GFKIDDKGMAPALRHLYKELMGPWN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |