Monoclonal Anti-PARK2 antibody produced in mouse
SIGMA/WH0005071M1 - clone 1H4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ARJP; Anti-PDJ; Anti-PRKN; Anti-Parkinson disease (autosomal recessive, juvenile) 2, parkin
MDL Number: MFCD02263896
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PARK2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting PARK2 monoclonal antibody, clone 1H4. Western Blot analysis of PARK2 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged PARK2 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC022014   |
| isotype | IgG3κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL |
| Biochem/physiol Actions: | Parkin RBR E3 ubiquitin protein ligase (PARK2) catalyzes the monoubiquitination and polyubiquitination of proteins to regulate various cellular processes. The encoded protein plays a vital role in synaptic regulation. Genetic variations in the gene leads to autosomal recessive Parkinson’s disease (AR-PD) and sporadic PD. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The parkin RBR E3 ubiquitin protein ligase (PARK2) gene, with 12 exons spanning 1.35Mb on genomic DNA, is mapped to human chromosome 6q26. The gene codes for E3 ubiquitin-protein ligase parkin. The protein contains conserved ubiquitin-like domain (UBL) at N- terminal and four zinc coordinating domains, such as, RING0, RING1, in between ring (IBR) and RING2 domains at C-terminal. |
| General description: | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. (provided by RefSeq) |
| Immunogen: | PARK2 (AAH22014, 288 a.a. ~ 387 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PCVGTGDTVVLRGALGGFRRGVAGC |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |