Monoclonal Anti-PCK1 antibody produced in mouse
SIGMA/WH0005105M1 - clone 3E4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC22652; Anti-PEPCK1; Anti-PEPCKC; Anti-
MDL Number: MFCD08460534
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PCK1 on HepG2 cell. [antibody concentration 10 μg/mL]
Western Blotting PCK1 monoclonal antibody, clone 3E4 Western Blot analysis of PCK1 expression in HepG2.
Western Blotting Western Blot analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody, clone 3E4. Lanes Lane 1: PCK1 transfected lysate (69.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (94.16 kDa).
Immunoprecipitation Immunoprecipitation of PCK1 transfected lysate using anti-PCK1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PCK1 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC023978   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P35558 |
| Biochem/physiol Actions: | PCK1 (phosphoenolpyruvate carboxykinase 1) participates in gluconeogenesis, glyceroneogenesis and cataplerosis. It transforms oxaloacetic acid (OAA) into phosphoenol pyruvate (PEP) with the help of guanosine triphosphate (GTP). PEPCK is linked with type 2 diabetes. |
| General description: | PCK1 (phosphoenolpyruvate carboxykinase 1) is an enzyme that is expressed in humans as two isoforms- cytosolic PEPCK (cPEPCK) and mitochondrial PEPCK (mPEPCK). PCK1 gene is located on human chromosome 20q13. |
| Immunogen: | PCK1 (AAH23978, 1 a.a. ~ 622 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPPQLQNGLNLSAKVVQGSLDSLPQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |