Monoclonal Anti-PCMT1 antibody produced in mouse
SIGMA/WH0005110M2 - clone 1D6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-
Product Type: Chemical
Western Blotting PCMT1 monoclonal antibody, clone 1D6. Western Blot analysis of PCMT1 expression in PC-12.
Western Blotting PCMT1 monoclonal antibody, clone 1D6 Western Blot analysis of PCMT1 expression in HeLa.
Western Blotting PCMT1 monoclonal antibody, clone 1D6. Western Blot analysis of PCMT1 expression in NIH/3T3.
Western Blotting PCMT1 monoclonal antibody, clone 1D6. Western Blot analysis of PCMT1 expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
ELISA Detection limit for recombinant GST tagged PCMT1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005389   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P22061 |
| General description: | Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM |
| Immunogen: | PCMT1 (NP_005380, 117 a.a. ~ 225 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence DDSVNNVRKDDPTLLSSGRVQLVVG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |