Monoclonal Anti-PCSK1 antibody produced in mouse
SIGMA/WH0005122M2 - clone 3D2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-NEC1; Anti-PC1; Anti-PC3; Anti-SPC3; Anti-proprotein convertase subtilisin/kexin type 1
MDL Number: MFCD03455801
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to PCSK1 on HeLa cell. [antibody concentration 30 μg/mL]
Western Blotting PCSK1 monoclonal antibody, clone 3D2 Western Blot analysis of PCSK1 expression in HeLa.
Western Blotting PCSK1 monoclonal antibody, clone 3D2. Western Blot analysis of PCSK1 expression in Raw 264.7.
Western Blotting PCSK1 monoclonal antibody, clone 3D2. Western Blot analysis of PCSK1 expression in NIH/3T3.
Western Blotting PCSK1 monoclonal antibody, clone 3D2. Western Blot analysis of PCSK1 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (36.96 kDa).
ELISA Detection limit for recombinant GST tagged PCSK1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_000439   |
| isotype | IgG3κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P29120 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. The use of alternate polyadenylation sites has been found for this gene. Multiple alternatively spliced transcript variants have been described for this gene but their full length nature is not known. (provided by RefSeq) |
| Immunogen: | PCSK1 (NP_000430, 652 a.a. ~ 753 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GGRRDELEEGAPSEAMLRLLQSAFS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |