Monoclonal Anti-PDGFRB antibody produced in mouse
SIGMA/WH0005159M8 - clone 4C12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CD140B; Anti-JTK12; Anti-PDGFR; Anti-PDGFR1; Anti-PDGFRbeta; Anti-
MDL Number: MFCD00164777
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB. Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PDGFRB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting PDGFRB monoclonal antibody, clone 4C12. Western Blot analysis of PDGFRB expression in human stomach.
Western Blotting PDGFRB monoclonal antibody, clone 4C12. Western Blot analysis of PDGFRB expression in human uterus myoma.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of PDGFRB transfected lysate using anti-PDGFRB monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PDGFRB rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PDGFRB is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC032224   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P09619 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. (provided by RefSeq) |
| Immunogen: | PDGFRB (AAH32224, 33 a.a. ~ 133 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LVVTPPGPELVLNVSSTFVLTCSGS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |