Monoclonal Anti-PECAM1 antibody produced in mouse
SIGMA/WH0005175M1 - clone 1D2-1A5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CD31; Anti-PECAM1; Anti-
MDL Number: MFCD01092362
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PECAM1 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma tissue. [antibody concentration 5 μg/mL]
Western Blotting Western Blot analysis of PECAM1 expression in transfected 293T cell line by PECAM1 monoclonal antibody, clone 1D2-1A5. Lanes Lane 1: PECAM1 transfected lysate (82.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting PECAM1 monoclonal antibody, clone 1D2-1A5. Western Blot analysis of PECAM1 expression in human placenta.
Western Blotting QC Western Blot detection against Immunogen (103.95 kDa).
Immunoprecipitation Immunoprecipitation of PECAM1 transfected lysate using anti-PECAM1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PECAM1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PECAM1 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D2-1A5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC022512   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P16284 |
| Biochem/physiol Actions: | Platelet and endothelial cell adhesion molecule 1 (PECAM1) also called as cluster of differentiation 31 (CD31), plays a crucial role in the adhesion cascade leading to leukocyte extravasation during the inflammatory process. PECAM1 is essential for leukocyte transmigration via intercellular junctions of vascular endothelial cells. Genetic polymorphism of PECAM1 is associated with atherosclerotic events. PECAM1, is a membrane glycoprotein that facilitates both homophilic and heterophilic adhesion. PECAM1 is also implicated in angiogenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The platelet and endothelial cell adhesion molecule 1 (PECAM1) gene, spanning 75-kb, is mapped on the long arm of human chromosome 17. This gene codes for 130kDa protein, which belongs to the immunoglobulin (Ig) superfamily. It is a vital component of the endothelial cell intercellular junction. PECAM1 is expressed on the surface of circulating platelets, monocytes, neutrophils, and selected T-cell subsets. |
| Immunogen: | PECAM1 (AAH22512, 29 a.a. ~ 738 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ENSFTINSVDMKSLPDWTVQNGKNL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |