Monoclonal Anti-PEPD antibody produced in mouse
SIGMA/WH0005184M1 - clone 1D5-H3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC10905; Anti-PROLIDASE; Anti-peptidase D
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10 μg/mL]
Western Blotting PEPD monoclonal antibody, clone 1D5-H3 Western Blot analysis of PEPD expression in HepG2.
Western Blotting Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody, clone 1D5-H3. Lanes Lane 1: PEPD transfected lysate (Predicted MW: 54.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (79.97 kDa).
Immunoprecipitation Immunoprecipitation of PEPD transfected lysate using anti-PEPD monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PEPD rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PEPD is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D5-H3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC015027   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P12955 |
| General description: | Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of iminoacids. (provided by RefSeq) |
| Immunogen: | PEPD (AAH15027, 1 a.a. ~ 493 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAATGPSFWLGNETLKVPLALFAL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |