Monoclonal Anti-PF4 antibody produced in mouse
SIGMA/WH0005196M1 - clone 3F6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CXCL4; Anti-MGC138298; Anti-Platelet factor 4 (chemokine (C-X-C motif) ligand 4); Anti-SCYB4
MDL Number: MFCD00282331
Product Type: Chemical
Western Blotting Western Blot analysis of PF4 expression in transfected 293T cell line by PF4 monoclonal antibody, clone 3F6. Lanes Lane 1: PF4 transfected lysate (10.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (33.55 kDa).
Immunoprecipitation Immunoprecipitation of PF4 transfected lysate using anti-PF4 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PF4 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PF4 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_002619   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P02776 |
| Biochem/physiol Actions: | Platelet factor-4 (PF-4) is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. This protein associates with heparin and neutralizes it. It also neutralizes various negatively charged proteoglycans. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM |
| Immunogen: | PF4 (NP_002610, 31 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence AEAEEDGDLQCLCVKTTSQVRPRHI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |