Monoclonal Anti-PITX1 antibody produced in mouse
SIGMA/WH0005307M1 - clone 5G4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BFT; Anti-POTX; Anti-PTX1; Anti-paired-like homeodomain transcription factor 1
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi. Blot probed with PITX1 monoclonal antibody, clone 5G4. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting PITX1 monoclonal antibody, clone 5G4 Western Blot analysis of PITX1 expression in HeLa S3 NE.
Western Blotting Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody, clone 5G4. Lanes Lane 1: PITX1 transfected lysate (34.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.53 kDa).
Immunoprecipitation Immunoprecipitation of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PITX1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PITX1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5G4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_002653   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P78337 |
| General description: | This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. (provided by RefSeq) |
| Immunogen: | PITX1 (NP_002644, 225 a.a. ~ 313 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPSSMGPGAVPGMPNSGLNNINNLT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |