Monoclonal Anti-PLK1 antibody produced in mouse
SIGMA/WH0005347M1 - clone 2G12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PLK; Anti-STPK13; Anti-polo-like kinase 1 (Drosophila)
MDL Number: MFCD00803562
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody, clone 2G12. Lanes Lane 1: PLK1 transfected lysate (68.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
Western Blotting PLK1 monoclonal antibody, clone 2G12 Western Blot analysis of PLK1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (91.85 kDa).
Immunoprecipitation Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PLK1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PLK1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC002369   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P53350 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | PLK1 (AAH02369, 1 a.a. ~ 603 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSAAVTAGKLARAPADPGKAGVPGV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |