Monoclonal Anti-PML antibody produced in mouse
SIGMA/WH0005371M2 - clone 1D12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MYL; Anti-PP8675; Anti-RNF71; Anti-TRIM19; Anti-promyelocytic leukemia
MDL Number: MFCD06412254
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between TP53 and PML. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting PML monoclonal antibody, clone 1D12 Western Blot analysis of PML expression in HeLa S3 NE.
Western Blotting PML monoclonal antibody, clone 1D12. Western Blot analysis of PML expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged PML is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC000080   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P29590 |
| Biochem/physiol Actions: | Promyelocytic leukemia (PML) helps in the complete formation of the nuclear body. It can serve as a transcriptional cofactor. PML plays major roles in modulating cell morphology, proliferation and migration. It has the capability to block the ubiquitination and proteasomal degradation processes. Cytoplasmic PML is required to control TGF-β1 (transforming growth factor β 1) signaling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Promyelocytic leukemia (PML) is a 70 KDa protein, made of 560 amino acids. PML is present in the nucleus. It has 9 coding exons and is mapped to human chromosome 15q24. |
| Immunogen: | PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RDPIDVDLDVSNTTTAQKRKCSQTQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |