Monoclonal Anti-POU3F2 antibody produced in mouse
SIGMA/WH0005454M1 - clone 6F6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-39728; Anti-BRN2; Anti-OTF7; Anti-POU domain, class 3, transcription factor 2; Anti-POUF3
Product Type: Chemical
Western Blotting Western Blot analysis of POU3F2 expression in transfected 293T cell line by POU3F2 monoclonal antibody, clone 6F6. Lanes Lane 1: POU3F2 transfected lysate (46.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting POU3F2 monoclonal antibody, clone 6F6. Western Blot analysis of POU3F2 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (33.11 kDa).
ELISA Detection limit for recombinant GST tagged POU3F2 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005604   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P20265 |
| General description: | POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM |
| Immunogen: | POU3F2 (NP_005595, 1 a.a. ~ 67 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MATAASNHYSLLTSSASIVHAEPPG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |