Monoclonal Anti-POU6F1 antibody produced in mouse
SIGMA/WH0005463M1 - clone 6H1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BRN5; Anti-MPOU; Anti-POU domain, class 6, transcription factor 1; Anti-TCFB1
Product Type: Chemical
Western Blotting Western Blot analysis of POU6F1 expression in transfected 293T cell line by POU6F1 monoclonal antibody, clone 6H1. Lanes Lane 1: POU6F1 transfected lysate (32.6 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of POU6F1 over-expressed 293 cell line, cotransfected with POU6F1 Validated Chimera RNAi. Blot probed with POU6F1 monoclonal antibody, clone 6H1. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting POU6F1 monoclonal antibody, clone 6H1 Western Blot analysis of POU6F1 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
ELISA Detection limit for recombinant GST tagged POU6F1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6H1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_002702   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q14863 |
| Immunogen: | POU6F1 (NP_002693, 193 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ITPKSAQKLKPVLEKWLNEAELRNQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |