Monoclonal Anti-PPARBP antibody produced in mouse
SIGMA/WH0005469M1 - clone 2A2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CRSP1; Anti-CRSP200; Anti-DRIP205; Anti-DRIP230; Anti-MED1; Anti-MGC71488; Anti-PBP; Anti-PPAR binding protein; Anti-PPARGBP; Anti-RB18A; Anti-TRAP220; Anti-TRIP2
MDL Number: MFCD05665239
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody, clone 2A2. Lanes Lane 1: PPARBP transfected lysate (61.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged PPARBP is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_004774   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15648 |
| General description: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. (provided by RefSeq) |
| Immunogen: | PPARBP (NP_004765, 1391 a.a. ~ 1490 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KIIISKHDGGSPSIKAKVTLQKPGE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |