Monoclonal Anti-PPM1B antibody produced in mouse
SIGMA/WH0005495M1 - clone 1A3-2A4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC21657; Anti-PP2CB; Anti-PP2CBETA; Anti-PP2CbetaX; Anti-PPC2BETAX; Anti-protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PPM1B on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between ARRB2 and PPM1B. HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 and anti-PPM1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Enhanced Validation-RNAi Western blot analysis of PPM1B over-expressed 293 cell line, cotransfected with PPM1B Validated Chimera RNAi. Blot probed with PPM1B monoclonal antibody, clone 1A3-2A4. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of PPM1B expression in transfected 293T cell line by PPM1B monoclonal antibody, clone 1A3-2A4. Lanes Lane 1: PPM1B transfected lysate (52.643 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (46.86 kDa).
Immunoprecipitation Immunoprecipitation of PPM1B transfected lysate using anti-PPM1B monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PPM1B monoclonal antibody.
ELISA Detection limit for recombinant GST tagged PPM1B is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A3-2A4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC012002   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O75688 |
| General description: | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. (provided by RefSeq) |
| Immunogen: | PPM1B (AAH12002, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSIVLVCFSNAPKVSDEAVKKDSEL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |