Monoclonal Anti-PPP3CB antibody produced in mouse
SIGMA/WH0005532M1 - clone 5D3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CALNA2; Anti-CALNB; Anti-protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)
MDL Number: MFCD00282169
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of PPP3CB over-expressed 293 cell line, cotransfected with PPP3CB Validated Chimera RNAi. Blot probed with PPP3CB monoclonal antibody, clone 5D3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of PPP3CB expression in transfected 293T cell line by PPP3CB monoclonal antibody, clone 5D3. Lanes Lane 1: PPP3CB transfected lysate (59.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.64 kDa).
ELISA Detection limit for recombinant GST tagged PPP3CB is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5D3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_021132   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P16298 |
| Immunogen: | PPP3CB (NP_066955, 435 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LTPTGMLPSGVLAGGRQTLQSATVE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |