Monoclonal Anti-PRKCI antibody produced in mouse
SIGMA/WH0005584M1 - clone 1G11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DXS1179E; Anti-MGC26534; Anti-PKCI; Anti-nPKCiota; Anti-protein kinase C, iota
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PRKCI on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody, clone 1G11. Lanes Lane 1: PRKCI transfected lysate (68.3 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of PRKCI over-expressed 293 cell line, cotransfected with PRKCI Validated Chimera RNAi. Blot probed with PRKCI monoclonal antibody (M01) clone 1G11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (90.31 kDa).
ELISA Detection limit for recombinant GST tagged PRKCI is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC022016   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P41743 |
| General description: | This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphat |
| Immunogen: | PRKCI (AAH22016, 1 a.a. ~ 587 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSHTVAGGGSGDHSHQVRVKAYYRG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |