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Monoclonal Anti-PKN1 antibody produced in mouse

SIGMA/WH0005585M1 - clone 1B10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-DBK; Anti-MGC46204; Anti-PAK1; Anti-PKN; Anti-PRK1; Anti-PRKCL1; Anti-protein kinase N1

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-WH0005585M1-100UG 100 µg
$524.00
1/EA
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Immunofluorescence Immunofluorescence of monoclonal antibody to PKN1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody, clone 1B10. Lanes Lane 1: PKN1 transfected lysate (103.9 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi. Blot probed with PKN1 monoclonal antibody (M01) clone 1B10. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (42.57 kDa).
Immunoprecipitation Immunoprecipitation of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PKN1 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged PKN1 is approximately 3 ng/mL as a capture antibody.

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone 1B10, monoclonal
conjugate unconjugated
form buffered aqueous solution
GenBank® accession no. BC040061 
isotype IgG2aκ
Quality Level 100 
shipped in dry ice
species reactivity human
storage temp. −20°C
technique(s) immunofluorescence: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 1-5 μg/mL
UniProt accession no. Q16512 
General description: The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. (provided by RefSeq)
Immunogen: PKN1 (AAH40061, 462 a.a. ~ 615 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
LDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDF
Legal Information: GenBank is a registered trademark of United States Department of Health and Human Services
Physical form: Solution in phosphate buffered saline, pH 7.4
RIDADR NONH for all modes of transport
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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