Monoclonal Anti-PKN1 antibody produced in mouse
SIGMA/WH0005585M1 - clone 1B10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DBK; Anti-MGC46204; Anti-PAK1; Anti-PKN; Anti-PRK1; Anti-PRKCL1; Anti-protein kinase N1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PKN1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody, clone 1B10. Lanes Lane 1: PKN1 transfected lysate (103.9 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi. Blot probed with PKN1 monoclonal antibody (M01) clone 1B10. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (42.57 kDa).
Immunoprecipitation Immunoprecipitation of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PKN1 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged PKN1 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC040061   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q16512 |
| General description: | The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. (provided by RefSeq) |
| Immunogen: | PKN1 (AAH40061, 462 a.a. ~ 615 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LDMEPQGCLVAEVTFRNPVIERIPR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |