Monoclonal Anti-PRKCZ antibody produced in mouse
SIGMA/WH0005590M1 - clone 2D1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PKC2; Anti-protein kinase C, zeta
MDL Number: MFCD00162775
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of PRKCZ over-expressed 293 cell line, cotransfected with PRKCZ Validated Chimera RNAi. Blot probed with PRKCZ monoclonal antibody (M01) clone 2D1. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of PRKCZ expression in transfected 293T cell line by PRKCZ monoclonal antibody, clone 2D1. Lanes Lane 1: PRKCZ transfected lysate (67.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.42 kDa).
ELISA Detection limit for recombinant GST tagged PRKCZ is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC008058   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q05513 |
| Biochem/physiol Actions: | PRKCZ (Protein kinase C ζ) is involved in a variety of cellular processes including cell proliferation, cell motility, and cell survival. It acts as a regulatory molecule in the insulin signaling pathways. It has been suggested that PRKCZ performs downstream of phosphatidylinositol 3-kinase (PI 3-kinase) in the insulin signal pathway for the translocation of GLUT4-encoded protein. It has also been reported that PRKCZ may act as a negative regulator by phosphorylating insulin receptor substrate-1 (IRS-1), which restricts its ability to activate phosphatidylinositol 3-kinase in response to insulin. It has been documented that PRKCZ might be involved in the pathogenesis of type 2 diabetes mellitus (T2DM). PRKCZ also has been reported to be part of various signaling pathways such as ERK/MAPK activation pathway, p70 ribosomal S6 kinase signaling cascade, activation of transcription factor NF-κB. It also plays a role in the regulation of cell polarity. Study predicts that PRKCZ may have a role in the ovarian cancer progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. (provided by RefSeq) |
| Immunogen: | PRKCZ (AAH08058, 165 a.a. ~ 255 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KLLVHKRCHGLVPLTCRKHMDSVMP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |