Monoclonal Anti-MAPK3 antibody produced in mouse
SIGMA/WH0005595M1 - clone 3C9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ERK1; Anti-P44ERK1; Anti-P44MAPK; Anti-PRKM3; Anti-
MDL Number: MFCD02179320
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MAPK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 25 μg/mL]
Western Blotting MAPK3 monoclonal antibody, clone 3C9 Western Blot analysis of MAPK3 expression in A-431.
Western Blotting Western Blot analysis of MAPK3 expression in transfected 293T cell line by MAPK3 monoclonal antibody, clone 3C9. Lanes Lane 1: MAPK3 transfected lysate (43.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of MAPK3 transfected lysate using anti-MAPK3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MAPK3 monoclonal antibody.
ELISA Detection limit for recombinant GST tagged MAPK3 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC013992   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P27361 |
| General description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. (provided by RefSeq) |
| Immunogen: | MAPK3 (AAH13992, 279 a.a. ~ 379 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence NYLQSLPSKTKVAWAKLFPKSDSKA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |