Monoclonal Anti-MAP2K5 antibody produced in mouse
SIGMA/WH0005607M1 - clone M1-E6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HsT17454; Anti-MAPKK5; Anti-MEK5; Anti-PRKMK5; Anti-
MDL Number: MFCD01633629
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MAP2K5 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting MAP2K5 monoclonal antibody, clone M1-E6. Western Blot analysis of MAP2K5 expression in K-562.
Western Blotting MAP2K5 monoclonal antibody, clone M1-E6 Western Blot analysis of MAP2K5 expression in Jurkat.
Western Blotting Western Blot analysis of MAP2K5 expression in transfected 293T cell line by MAP2K5 monoclonal antibody, clone M1-E6. Lanes Lane 1: MAP2K5 transfected lysate (Predicted MW: 50.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (75.02 kDa).
ELISA Detection limit for recombinant GST tagged MAP2K5 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M1-E6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC008838   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13163 |
| General description: | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. (provided by RefSeq) |
| Immunogen: | MAP2K5 (AAH08838, 1 a.a. ~ 448 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MLWLALGPFPAMENQVLVIRIKIPN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |