Monoclonal Anti-PSAP antibody produced in mouse
SIGMA/WH0005660M1 - clone 1D1-C12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ00245; Anti-GLBA; Anti-MGC110993; Anti-SAP1; Anti-prosaposin; Anti-prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy)
MDL Number: MFCD00163953
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PSAP on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 5 μg/mL]
Western Blotting Western Blot analysis of PSAP expression in transfected 293T cell line by PSAP monoclonal antibody, clone 1D1-C12. Lanes Lane 1: PSAP transfected lysate (58.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (81.29 kDa).
Immunoprecipitation Immunoprecipitation of PSAP transfected lysate using anti-PSAP monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PSAP rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D1-C12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC001503   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P07602 |
| Biochem/physiol Actions: | Prosaposin (PSAP) activates the androgen receptor and has been linked to stimulate metastasis in neoplasms. A variant in the PSAP gene leads to prosaposin deficiency which is a fatal infantile lysosomal storage disorder. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Secreted prosaposin (PSAP) is a pleiotropic growth factor. It is found in bile, seminal plasma, human milk and cerebrospinal fluid. It also exists as a lysosomal protein. In lysosomes, PSAP is the precursor of four sphingolipid activator proteins (saposin A–D). The gene encoding PSAP is localized on human chromosome 10q22.1. |
| Immunogen: | PSAP (AAH01503, 18 a.a. ~ 524 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PVLGLKECTRGSAVWCQNVKTASDC |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |