Monoclonal Anti-PSMA7 antibody produced in mouse
SIGMA/WH0005688M1 - clone 1A10-3G12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-C6; Anti-HSPC; Anti-MGC3755; Anti-RC61; Anti-XAPC7; Anti-proteasome (prosome, macropain) subunit, alpha type, 7
MDL Number: MFCD00162194
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 5 μg/mL]
Immunohistochemistry Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10 μg/mL]
Western Blotting Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody, clone 1A10-3G12. Lanes Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting PSMA7 monoclonal antibody, clone 1A10-3G12 Western Blot analysis of PSMA7 expression in HeLa.
Western Blotting PSMA7 monoclonal antibody, clone 1A10-3G12. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.
Western Blotting QC Western Blot detection against Immunogen (53.02 kDa).
Immunoprecipitation Immunoprecipitation of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PSMA7 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PSMA7 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A10-3G12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC004427   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O14818 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9. (provided by RefSeq) |
| Immunogen: | PSMA7 (AAH04427, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSYDRAITVFSPDGHLFQVEYAQEA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |