Monoclonal Anti-PTPRN2 antibody produced in mouse
SIGMA/WH0005799M8 - clone 7F8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-IA2beta; Anti-IAR; Anti-ICAAR; Anti-PTPRP; Anti-protein tyrosine phosphatase, receptor type, N polypeptide 2
Product Type: Chemical
Western Blotting Western Blot analysis of PTPRN2 expression in transfected 293T cell line by PTPRN2 monoclonal antibody, clone 7F8. Lanes Lane 1: PTPRN2 transfected lysate (108.1 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of PTPRN2 over-expressed 293 cell line, cotransfected with PTPRN2 Validated Chimera RNAi. Blot probed with PTPRN2 monoclonal antibody, clone 7F8. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (37.29 kDa).
ELISA Detection limit for recombinant GST tagged PTPRN2 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7F8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_002847   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q92932 |
| General description: | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. The catalytic domain of this PTP is most closely related to PTPRN/IA-2beta. This PTP and PTPRN are both found to be major autoantigens associated with insulin-dependent diabetes mellitus. Three alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. (provided by RefSeq) |
| Immunogen: | PTPRN2 (NP_002838, 108 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ELADLPKTYLRRPEASSPARPSKHS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |