Monoclonal Anti-RAB27A antibody produced in mouse
SIGMA/WH0005873M2 - clone 1G7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-GS2; Anti-HsT18676; Anti-MGC117246; Anti-RAB27; Anti-RAB27A, member RAS oncogene family; Anti-RAM
MDL Number: MFCD01634073
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RAB27A on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of RAB27A expression in transfected 293T cell line by RAB27A monoclonal antibody, clone 1G7. Lanes Lane 1: RAB27A transfected lysate (24.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting RAB27A monoclonal antibody, clone 1G7 Western Blot analysis of RAB27A expression in HL-60.
Enhanced Validation-RNAi Western blot analysis of RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi. Blot probed with RAB27A monoclonal antibody, clone 1G7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged RAB27A is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_004580   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51159 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. (provided by RefSeq) |
| Immunogen: | RAB27A (NP_004571, 122 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence YCENPDIVLCGNKSDLEDQRVVKEE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |