Monoclonal Anti-RAD51C antibody produced in mouse
SIGMA/WH0005889M1 - clone 3F3-5C6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-RAD51 homolog C (S. cerevisiae); Anti-RAD51L2
Product Type: Chemical
Western Blotting Western Blot analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody, clone 3F3-5C6. Lanes Lane 1: RAD51C transfected lysate (42.2 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi. Blot probed with RAD51C monoclonal antibody, clone 3F3-5C6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting RAD51C monoclonal antibody, clone 3F3-5C6 Western Blot analysis of RAD51C expression in HeLa.
Western Blotting RAD51C monoclonal antibody, clone 3F3-5C6. Western Blot analysis of RAD51C expression in 293.
Western Blotting QC Western Blot detection against Immunogen (40.48 kDa).
ELISA Detection limit for recombinant GST tagged RAD51C is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F3-5C6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC000667   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43502 |
| Biochem/physiol Actions: | RAD51 paralog C (RAD51C) aids in the recruitment of the enzyme RAD51 to DNA break sites. It may be involved in modulating its translocation from the cytoplasm to the nucleus. RAD51C has a role in genomic stability and homologous recombination repair. It has been associated with breast and ovarian cancer. Mutations in the gene encoding RAD51C have been linked to Fanconi anemia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | RAD51 paralog C (RAD51C) is a tumor suppressor gene. It belongs to the RAD51-like gene family and the gene encoding it is localized on human chromosome 17q23. |
| Immunogen: | RAD51C (AAH00667.1, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRGKTFRFEMQRDLVSFPLSPAVRV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |