Monoclonal Anti-RALB antibody produced in mouse
SIGMA/WH0005899M4 - clone 4D1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
MDL Number: MFCD01634081
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RALB on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of RALB expression in transfected 293T cell line by RALB monoclonal antibody, clone 4D1. Lanes Lane 1: RALB transfected lysate (23.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.08 kDa).
Immunoprecipitation Immunoprecipitation of RALB transfected lysate using anti-RALB monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with RALB rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged RALB is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC018163   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P11234 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. (provided by RefSeq) |
| Immunogen: | RALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FLLVFSITEHESFTATAEFREQILR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |