Monoclonal Anti-RGS2 antibody produced in mouse
SIGMA/WH0005997M1 - clone 4C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-G0S8; Anti-regulator of G-protein signalling 2, 24kDa
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 μg/mL]
Western Blotting RGS2 monoclonal antibody, clone 4C4 Western Blot analysis of RGS2 expression in MCF-7.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged RGS2 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC007049   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P41220 |
| Biochem/physiol Actions: | Regulator of G-protein signaling 2 (RGS2) or G0/G1 switch regulatory gene 8 (GOS8) can decrease the activity of G protein through its GTPase actions. It acts as a GTPase activating protein (GAP) for G-protein (Gi). It might bind to adenylate cyclase to play an inhibitory role in Gs-mediated adenylate cyclase activity. RGS2 can block the proliferation of androgen-independent prostate cancer cells by inhibiting both constitutively activated extracellular signal-regulated kinases (ERKs) and androgen-independent androgen receptor (AR) activity. RGS2 is expressed at a high level in several solid breast cancers. Variation in the RGS2 gene might be involved in the development of anxiety in humans. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. (provided by RefSeq) |
| General description: | Regulator of G-protein signaling 2 (RGS2) or G0/G1 switch regulatory gene 8 (GOS8) protein belongs to the regulators of G-protein signaling (RGS) protein family of GTPase accelerating proteins (GAPs). Its mRNA expression is mainly seen in the brain, heart, lung, placenta, kidney, intestine, lymphocytes, and testis. RGS2 is located in the nucleus and the plasma membrane. RGS2 gene is located on human chromosome 1q31.2. |
| Immunogen: | RGS2 (AAH07049, 111 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EFWLACEDFKKTKSPQKLSSKARKI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |