Monoclonal Anti-RNF2 antibody produced in mouse
SIGMA/WH0006045M1 - clone 6C2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BAP1; Anti-DING; Anti-HIPI3; Anti-RING1B; Anti-RING2; Anti-ring finger protein 2
Product Type: Chemical
Western Blotting Western Blot analysis of RNF2 expression in transfected 293T cell line by RNF2 monoclonal antibody, clone 6C2. Lanes Lane 1: RNF2 transfected lysate (37.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting RNF2 monoclonal antibody, clone 6C2 Western Blot analysis of RNF2 expression in NIH/3T3.
Western Blotting RNF2 monoclonal antibody, clone 6C2. Western Blot analysis of RNF2 expression in Raw 264.7.
Enhanced Validation-RNAi Western blot analysis of RNF2 over-expressed 293 cell line, cotransfected with RNF2 Validated Chimera RNAi. Blot probed with RNF2 monoclonal antibody, clone 6C2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged RNF2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6C2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_007212   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9CQJ4 |
| General description: | Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. (provided by RefSeq) |
| Immunogen: | RNF2 (NP_009143, 192 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PSNKRTKTSDDSGLELDNNNAAMAI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |