Monoclonal Anti-MRPL12 antibody produced in mouse
SIGMA/WH0006182M1 - clone 3B12-1A3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-5c52; Anti-L12mt; Anti-MGC8610; Anti-MRPL31/34; Anti-MRPL7; Anti-MRPL7/L12; Anti-RPML12; Anti-mitochondrial ribosomal protein L12
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody, clone 3B12-1A3. Lanes Lane 1: MRPL12 transfected lysate (21.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting MRPL12 monoclonal antibody, clone 3B12-1A3 Western Blot analysis of MRPL12 expression in COLO 320 HSR.
Enhanced Validation-RNAi Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi. Blot probed with MRPL12 monoclonal antibody, clone 3B12-1A3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (47.52 kDa).
ELISA Detection limit for recombinant GST tagged MRPL12 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B12-1A3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC002344   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P52815 |
| Biochem/physiol Actions: | MRPL12 (mitochondrial ribosomal protein) is involved in the mitochondrial translation. It directly interacts with the mitochondrial RNA polymerase (POLRMT) and triggers the mitochondrial transcriptional process. Research shows that the interaction between MRPL12 and POLRMT indicates its participation in the regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. Study shows that MRPL12 may be involved in the differentiation of colonic epithelial cells and its reduced mitochondrial activity have been shown to be linked with tumor formation. It has been reported that mutated expression of MRPL12 associates with the growth retardation, neurological deterioration and mitochondrial translation deficiency. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | MRPL12 (AAH02344, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MLPAAARPLWGPCLGLRAAAFRLAR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |