Monoclonal Anti-RPS6KA2 antibody produced in mouse
SIGMA/WH0006196M1 - clone 1F6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-HU2; Anti-MAPKAPK1C; Anti-RSK; Anti-RSK3; Anti-S6Kalpha; Anti-S6Kalpha2; Anti-p90RSK2; Anti-pp90RSK3; Anti-ribosomal protein S6 kinase, 90kDa, polypeptide 2
MDL Number: MFCD00676462
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between MAPK3 and RPS6KA2. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Enhanced Validation-RNAi Western blot analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi. Blot probed with RPS6KA2 monoclonal antibody (M01) clone 1F6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody, clone 1F6. Lanes Lane 1: RPS6KA2 transfected lysate (83.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting RPS6KA2 monoclonal antibody, clone 1F6 Western Blot analysis of RPS6KA2 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (37.07 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC002363   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15349 |
| General description: | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. (provided by RefSeq) |
| Immunogen: | RPS6KA2 (AAH02363, 631 a.a. ~ 733 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence YALSGGNWDSISDAAKDVVSKMLHV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |