Monoclonal Anti-RPS6KB1 antibody produced in mouse
SIGMA/WH0006198M1 - clone 1E12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-PS6K; Anti-S6K; Anti-S6K1; Anti-STK14A; Anti-p70(S6K)alpha; Anti-p70S6K; Anti-p70alpha; Anti-ribosomal protein S6 kinase, 70kDa, polypeptide 1
MDL Number: MFCD01092452
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RPS6KB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to RPS6KB1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting RPS6KB1 monoclonal antibody, clone 1E12 Western Blot analysis of RPS6KB1 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_003161   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P23443 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. (provided by RefSeq) |
| Immunogen: | RPS6KB1 (NP_003152, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PSVLESVKEKFSFEPKIRSPRRFIG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |