Monoclonal Anti-RRAS antibody produced in mouse
SIGMA/WH0006237M1 - clone 2E12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-related RAS viral (r-ras) oncogene homolog
Product Type: Chemical
Western Blotting RRAS monoclonal antibody, clone 2E12 Western Blot analysis of RRAS expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
Immunoprecipitation Immunoprecipitation of RRAS transfected lysate using anti-RRAS monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with RRAS rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged RRAS is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC016286   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P10301 |
| Application: | Monoclonal Anti-RRAS antibody produced in mouse has been used in Western Blotting. |
| Biochem/physiol Actions: | Ras related protein (RRAS) modulates cell migration, adhesion, differentiation and apoptosis. It acts as a molecular switch in signaling pathways. RRAS also activates integrins and subsequently leads to increase in matrix assembly and cell adhesion. The protein has been associated with breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ras related protein (RRAS) is part of the Ras superfamily of small GTPases. It is expressed in many tissues. The gene encoding RRAS is localized on human chromosome 19q13.3. |
| Immunogen: | RRAS (AAH16286, 109 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence AINDRQSFNEVGKLFTQILRVKDRD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |