Monoclonal Anti-SCP2 antibody produced in mouse
SIGMA/WH0006342M1 - clone 2E9-1B3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp686C12188; Anti-DKFZp686D11188; Anti-NLTP; Anti-NSLTP; Anti-SCPX; Anti-sterol carrier protein 2
Product Type: Chemical
Western Blotting Western Blot analysis of SCP2 expression in transfected 293T cell line by SCP2 monoclonal antibody, clone 2E9-1B3. Lanes Lane 1: SCP2 transfected lysate (Predicted MW: 35 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (41.47 kDa).
Immunoprecipitation Immunoprecipitation of SCP2 transfected lysate using anti-SCP2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with SCP2 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged SCP2 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E9-1B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC005911   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P22307 |
| General description: | This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. The full-length nature of all transcript variants has not been determined. (provided by RefSeq) |
| Immunogen: | SCP2 (AAH05911, 1 a.a. ~ 143 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGFPEAASSFRTHQIEAVPTSSASD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |