Monoclonal Anti-SDCBP antibody produced in mouse
SIGMA/WH0006386M1 - clone 2C12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MDA9; Anti-ST1; Anti-SYCL; Anti-TACIP18; Anti-syndecan binding protein (syntenin)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SDCBP on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SDCBP on HepG2 cell. [antibody concentration 35 μg/mL]
Western Blotting Western Blot analysis of SDCBP expression in transfected 293T cell line by SDCBP monoclonal antibody, clone 2C12. Lanes Lane 1: SDCBP transfected lysate (32.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting SDCBP monoclonal antibody, clone 2C12 Western Blot analysis of SDCBP expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of SDCBP transfected lysate using anti-SDCBP monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with SDCBP rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged SDCBP is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005625   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O00560 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. (provided by RefSeq) |
| Immunogen: | SDCBP (NP_005616, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSLYPSLEDLKVDKVIQAQTAFSAN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |