Monoclonal Anti-SFPQ antibody produced in mouse
SIGMA/WH0006421M2 - clone 6D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-POMP100; Anti-PSF; Anti-splicing factor proline/
MDL Number: MFCD03457573
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SFPQ on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SFPQ on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting SFPQ monoclonal antibody, clone 6D7 Western Blot analysis of SFPQ expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (35.97 kDa).
ELISA Detection limit for recombinant GST tagged SFPQ is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_005066   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P23246 |
| Application: | Monoclonal Anti-SFPQ antibody produced in mouse has been used in Western Blotting and immunofluorescence. |
| Biochem/physiol Actions: | Splicing factor proline and glutamine rich (SFPQ), along with its binding partner non-POU domain-containing octamer-binding protein (NONO/p54nrb), plays a vital role in RNA processing, RNA splicing and transcriptional regulation. Additionally, these proteins also play a regulatory role in selective nuclear retention of defective mRNAs. SFPQ participates in transcription repression by recruiting transcription regulator proteins Sin3a and histone deacetylase (HDAC). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Splicing factor proline and glutamine rich (SFPQ), also known as polypyrimidine tract-binding protein-associated-splici |
| Immunogen: | SFPQ (NP_005057, 269 a.a. ~ 361 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EEKISDSEGFKANLSLLRRPGEKTY |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |