Monoclonal Anti-SGK antibody produced in mouse
SIGMA/WH0006446M1 - clone 4D7-G3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-SGK1; Anti-
MDL Number: MFCD02686615
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5 μg/mL]
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10 μg/mL]
Enhanced Validation-RNAi Western blot analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi. Blot probed with SGK monoclonal antibody (M01) clone 4D7-G3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody, clone 4D7-G3. Lanes Lane 1: SGK transfected lysate (48.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (73.04 kDa).
ELISA Detection limit for recombinant GST tagged SGK is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D7-G3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC001263   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O00141 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. (provided by RefSeq) |
| Immunogen: | SGK (AAH01263.1, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTVKTEAAKGTLTYSRMRGMVAILI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |