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Monoclonal Anti-SKP1A antibody produced in mouse

SIGMA/WH0006500M1 - clone 1H8, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-EMC19; Anti-MGC34403; Anti-OCP2; Anti-OCPII; Anti-S-phase kinase-associated protein 1A (p19A); Anti-SKP1; Anti-TCEB1L

MDL Number: MFCD01633948
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-WH0006500M1-100UG 100 µg
$0.00
1/EA
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Immunofluorescence Immunofluorescence of monoclonal antibody to SKP1A on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting SKP1A monoclonal antibody, clone 1H8 Western Blot analysis of SKP1A expression in HeLa.
Western Blotting Western Blot analysis of SKP1 expression in transfected 293T cell line by SKP1A monoclonal antibody, clone 1H8. Lanes Lane 1: SKP1 transfected lysate (18.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.62 kDa).

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone 1H8, monoclonal
conjugate unconjugated
form buffered aqueous solution
GenBank® accession no. NM_006930 
isotype IgG2aκ
Quality Level 100 
shipped in dry ice
species reactivity human
storage temp. −20°C
technique(s) indirect ELISA: suitable
  indirect immunofluorescence: suitable
  western blot: 1-5 μg/mL
UniProt accession no. P63208 
General description: This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. (provided by RefSeq)
Immunogen: SKP1A (NP_008861, 53 a.a. ~ 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
AILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVGSTQFCL
Legal Information: GenBank is a registered trademark of United States Department of Health and Human Services
Physical form: Solution in phosphate buffered saline, pH 7.4
RIDADR NONH for all modes of transport
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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