Monoclonal Anti-SOX9 antibody produced in mouse
SIGMA/WH0006662M2 - clone 3C10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CMD1; Anti-CMPD1; Anti-SRA1; Anti-SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody, clone 3C10. Lanes Lane 1: SOX9 transfected lysate (56.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting SOX9 monoclonal antibody, clone 3C10 Western Blot analysis of SOX9 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with SOX9 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged SOX9 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_000346   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P48436 |
| Application: | Monoclonal Anti-SOX9 antibody produced in mouse has been used in immunofluorescence. |
| Biochem/physiol Actions: | Sex determining region Y-box 9 (SOX9), a transcription factor, is associated with the testis-determining factor sex determining region Y (SRY). It is expressed mainly in adult tissues and also in fetal testis and skeletal tissue. SOX9 consists of two functional domains: a high-mobility group (HMG) DNA-binding domain and a C-terminal transactivation domain. It plays a major role in cartilage differentiation and early testis development. It has been reported that SOX9 might play a role in chondrogenesis. Mutation of SOX9 gene in human causes campomelic dysplasia, a severe dwarfism syndrome and autosomal XY sex reversal. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. (provided by RefSeq) |
| Immunogen: | SOX9 (NP_000337, 400 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EQLSPSHYSEQQQHSPQQIAYSPFN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |