Monoclonal Anti-AKR1D1 antibody produced in mouse
SIGMA/WH0006718M3 - clone 1C2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-3o5bred; Anti-SRD5B1; Anti-aldo-keto reductase family 1, member D1 (delta 4-
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of AKR1D1 over-expressed 293 cell line, cotransfected with AKR1D1 Validated Chimera RNAi. Blot probed with AKR1D1 monoclonal antibody, clone 1C2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody, clone 1C2. Lanes Lane 1: AKR1D1 transfected lysate (37.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged AKR1D1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_005989   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51857 |
| General description: | The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. (provided by RefSeq) |
| Immunogen: | AKR1D1 (NP_005980, 227 a.a. ~ 326 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence NPIWVNVSSPPLLKDALLNSLGKRY |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |