Monoclonal Anti-SSB antibody produced in mouse
SIGMA/WH0006741M1 - clone 1D6-H5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Sjogren syndrome antigen B (autoantigen La)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SSB on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 μg/mL]
Western Blotting SSB monoclonal antibody, clone 1D6-H5 Western Blot analysis of SSB expression in HL-60.
Western Blotting QC Western Blot detection against Immunogen (70.62 kDa).
ELISA Detection limit for recombinant GST tagged SSB is 10 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D6-H5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC001289   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P05455 |
| Biochem/physiol Actions: | Sjogren syndrome antigen B (SSB) plays a vital role in various stages of the RNA metabolism such as RNA polymerase III protection from exonuclease digestion, processing of specific cellular and viral RNA precursors, serving as an RNA chaperone, affecting telomere homeostasis and stimulating microRNA expression. It promotes proliferation, migration and invasion of cancer cells in vitro . In addition, it also enhances tumor growth in vivo. Overexpression of the gene has been observed in various types of cancers. |
| General description: | The Sjogren syndrome antigen B (SSB) gene with 11 exons contains two alternative promoters 1 (P1) and 1′ (P1′) coding for SSB protein. The gene is mapped to human chromosome 2q31.1. The gene coded for a RNA-binding protein that is ubiquitously expressed. |
| Immunogen: | SSB (AAH01289, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAENGDNEKMAALEAKICHQIEYYF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |