Monoclonal Anti-SSR4 antibody produced in mouse
SIGMA/WH0006748M1 - clone 2D3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TRAPD; Anti-signal sequence receptor, delta (translocon-
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to SSR4 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of SSR4 expression in transfected 293T cell line by SSR4 monoclonal antibody, clone 2D3. Lanes Lane 1: SSR4 transfected lysate (19 kDa). Lane 2: Non-transfected lysate.
Western Blotting SSR4 monoclonal antibody, clone 2D3 Western Blot analysis of SSR4 expression in C32.
Western Blotting QC Western Blot detection against Immunogen (42.24 kDa).
ELISA Detection limit for recombinant GST tagged SSR4 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC003371   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51571 |
| General description: | SSR4, also called TRAPD, is assumed to be involved in protein secretion. It is located in the Xq28 region, arranged in a compact head-to-head manner with the IDH3G gene. These two genes are driven by a bidirectional promoter located between them, and encode proteins involved in unrelated biochemical pathways located in different compartments of the cell. The nontranscribed intergenic region represents only 133 bp and is embedded in a CpG island. The CpG island functions as a bidirectional promoter to initiate the transcription of both functionally unrelated genes with distinct expression patterns. SSR4 consists of six exons and is approximately 70 kb telomeric to the ALD gene. Although alternative splicing of exon 5 has not been detected in human SSR4, transcript variants missing the region homologous to human exon 5 have been detected in both Xenopus laevis and Mus musculus. (provided by RefSeq) |
| Immunogen: | SSR4 (AAH03371, 24 a.a. ~ 173 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EACLEPQITPSYYTTSDAVISTETV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |