Monoclonal Anti-STAR antibody produced in mouse
SIGMA/WH0006770M1 - clone 5F9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-STARD1; Anti-steroidogenic acute regulator
Product Type: Chemical
Western Blotting Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody, clone 5F9. Lanes Lane 1: STAR transfected lysate (31.9 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi. Blot probed with STAR monoclonal antibody, clone 5F9. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged STAR is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5F9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC010550   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49675 |
| General description: | The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. (provided by RefSeq) |
| Immunogen: | STAR (AAH10550, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EAMQKALGILSNQEGWKKESQQDNG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |