Monoclonal Anti-SUOX antibody produced in mouse
SIGMA/WH0006821M1 - clone 1H1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-sulfite oxidase
Product Type: Chemical
Western Blotting Western Blot analysis of SUOX expression in transfected 293T cell line by SUOX monoclonal antibody, clone 1H1. Lanes Lane 1: SUOX transfected lysate (53.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting SUOX monoclonal antibody, clone 1H1 Western Blot analysis of SUOX expression in HepG2.
Enhanced Validation-RNAi Western blot analysis of SUOX over-expressed 293 cell line, cotransfected with SUOX Validated Chimera RNAi. Blot probed with SUOX monoclonal antibody, clone 1H1. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (79.42 kDa).
ELISA Detection limit for recombinant GST tagged SUOX is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC065193   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51687 |
| General description: | Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins. (provided by RefSeq) |
| Immunogen: | SUOX (AAH65193, 1 a.a. ~ 488 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGTLLGLGAVLAYQDHRCRAAQEST |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |