Monoclonal Anti-VAMP1 antibody produced in mouse
SIGMA/WH0006843M2 - clone 5A4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp686H12131; Anti-SYB1; Anti-
MDL Number: MFCD03097356
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to VAMP1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1.5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to VAMP1 on HepG2 cell. [antibody concentration 10 μg/mL]
Western Blotting VAMP1 monoclonal antibody, clone 5A4 Western Blot analysis of VAMP1 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (33.33 kDa).
ELISA Detection limit for recombinant GST tagged VAMP1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5A4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_014231   |
| isotype | IgG2aλ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P23763 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. (provided by RefSeq) |
| Immunogen: | VAMP1 (NP_055046, 28 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTSNRRLQQTQAQVEEVVDIIRVNV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |