Monoclonal Anti-TAF7 antibody produced in mouse
SIGMA/WH0006879M1 - clone 2C5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TAF2F; Anti-TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa; Anti-TAFII55
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1 μg/mL]
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to TAF7 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of TAF7 expression in transfected 293T cell line by TAF7 monoclonal antibody, clone 2C5. Lanes Lane 1: TAF7 transfected lysate (40.3 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi. Blot probed with TAF7 monoclonal antibody, clone 2C5. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting TAF7 monoclonal antibody, clone 2C5 Western Blot analysis of TAF7 expression in MCF-7.
Western Blotting QC Western Blot detection against Immunogen (36.08 kDa).
ELISA Detection limit for recombinant GST tagged TAF7 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC032737   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15545 |
| General description: | The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II. (provided by RefSeq) |
| Immunogen: | TAF7 (AAH32737, 130 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FIWNHGITLPLKNVRKRRFRKTAKK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |