Monoclonal Anti-MAP3K7 antibody produced in mouse
SIGMA/WH0006885M2 - clone 2E10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TAK1; Anti-TGF1a; Anti-
MDL Number: MFCD02097469
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of MAP3K7 over-expressed 293 cell line, cotransfected with MAP3K7 Validated Chimera RNAi. Blot probed with MAP3K7 monoclonal antibody (M02) clone 2E10. GAPDH (36.1 kDa) used as specificity and loading control.
Enhanced Validation-RNAi Western blot analysis of MAP3K7 over-expressed 293 cell line, cotransfected with MAP3K7 Validated Chimera RNAi. Blot probed with MAP3K7 monoclonal antibody (M02) clone 2E10. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of MAP3K7 expression in transfected 293T cell line by MAP3K7 monoclonal antibody, clone 2E10. Lanes Lane 1: MAP3K7 transfected lysate (63.8 kDa). Lane 2: Non-transfected lysate.
ELISA Detection limit for recombinant GST tagged MAP3K7 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC017715   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43318 |
| General description: | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. (provided by RefSeq) |
| Immunogen: | MAP3K7 (AAH17715, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSTASAASSSSSSSAGEMIEAPSQV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |